Edaravone significantly downregulated AQP4 and AQP9 mRNA and protein levels after CIRI, again implying that AQP4 and AQP9 work together to cause brain edema. Owing to the complex and multifaceted nature of cerebral ischemic stroke, sequential staging of therapy by a combination of different mechanistic approaches will probably prove most useful in stroke management. Nowadays, acupuncture has gained increasing popularity in modern health care, garnering new support from a myriad of scientific investigators. The popular use of acupuncture has also inspired many scientists to explore ancient traditional medical technology alongside conventional medicine. Although the precise manner in which acupuncture functions is unknown and requires further study, many researchers now claim that acupuncture and electroacupuncture are beneficial for treating ischemic stroke. Indeed, the assorted effects of acupuncture and electroacupuncture on the generation and blockade of free radicals, intracellular calcium, inflammationrelated cytokines, and/or vasogenic edema have all been proposed to explain their possible mechanisms of therapeutic action. The list of acupoints affecting the various meridians of the body is quite long, but the Baihui, Zusanli, Neiguan, Weizhong, Sanyinjiao, Chize, Renzhong, and Waiguan locations are the most frequently chosen acupoints. Special acupuncture manipulations are also clinically used to improve self-care ability and quality of life in ischemic stroke patients, including scalp acupuncture and resuscitating acupuncture. Traditional Chinese Medicine theory holds that GV20 belongs to the governing vessel, which in humans is located on the top of the head at the intersection of the middle sagittal line and the connection of the two ear apexes. GV20 functions to collect the yang around the body. After stimulation of GV20, the local yang is dispersed over and energizes the entire body. ST36 is located at 3 cm below Dubi and one finger’s breadth before the anterior crest of the tibia, and is utilized as an acupoint for treating digestive system diseases. ST36 is one of several acupoints of the stomach meridian, which is rich in both Qi and blood, and thus stimulation at ST36 has the capacity to modulate the function of the entire body. Once Qi and blood are enriched, the body can again be activated. Of relevance to the current study, simultaneous stimulation of GV20 and ST60 reportedly has a synergistically beneficial effect on the attenuation of brain ischemia. Electroacupuncture, which can deliver continuous stimulation to acupoints, is currently under investigation for the management of ischemic stroke in experimental animals and in clinical practice. Previous work compared the therapeutic efficacy of acupuncture at ST36 and GV20 vs. electroacupuncture at ST36 and GV20 in rats undergoing CIRI and suggested that both treatments significantly increased hippocampal cell proliferation relative to the control.