The vulnerability of the interaction networks to targeted perturbations of highly connected hubs may explain why mutations of these critical hot spot residues could simultaneously disrupt many interactions leading a significant loss in chaperone activity. The results of this study suggest that the topology of the interaction networks may be determined by the structural architecture of the Hsp90 complexes and functionally Ganoderic-acid-F important changes that direct the Tubuloside-A ATPase cycle are often coordinated by a small number of highly connected conserved hubs. Holmium laser enucleation of the prostate is a newer surgical treatment of benign prostatic hyperplasia that was introduced in 1995. It involves enucleation and morcellation procedures. HoLEP enables any size of prostate to be treated in a minimally invasive manner. Many authors have reported that HoLEP is as effective as the transurethral resection of prostate with much shorter duration of urethral catheterization. However, sometimes after urethral catheter removal, clinicians encounter urinary retention resulting in the need for a re-catheterization due to a voiding failure. To avoid a bladder injury during morcellation, it is required to keep the bladder distended. Therefore, due to over-distention of the bladder, there is a concern for myogenic injury of the bladder that is responsible for de novo UR, despite a successful relief of a bladder outlet obstruction. However, no report has been previously published on de novo UR after HoLEP, however, a few reports have mentioned de novo UR is a postoperative complication of prostatectomy. This study was undertaken to describe the characteristics of de novo UR, and to identify independent risk factors that influence UR. Over recent decades, many authors have demonstrated the efficacy, safety, and indications of HoLEP in LUTS/BPH as compared with other surgical procedures. The advantages of HoLEP, such as, the absence of TUR syndrome, better hemostatic properties, lower perioperative morbidity, and shorter hospital stay are well established. Recently, HoLEP has been increasingly regarded as a new gold standard for treatment of LUTS/BPH. However, HoLEP still has its limitations, which can include a steep learning curve, diverse intraoperative, and early postoperative complications.Failure to void after surgery is a difficult situation for both patients and clinicians. This study was designed to identify risk factors in UR patients after HoLEP by comparing these patients with non-UR patients, as knowledge of these factors might enable us to better understand the natural history, as well as risk factors, of UR. Previous studies have shown that complication rates are correlated with surgeon’s experience. However, in the present study, surgical experience was not found to influence UR. Bladder over-distention has been previously reported to result in myogenic failure and detrusor instability in BPH patients. In addition, some researchers claimed that the detrusor instability in diabetic patients and the detrusor underactivity in BPH patients have also been associated with incidence of UR. In this study, a history of diabetes was not associated with the UR. The bladder is distended to greater than maximal bladder capacity during the morcellation of enucleated prostatic nodule for an average morcellating time of 11.3 minutes and this is likely to adversely affect the bladder detrusor and result in postoperative voiding difficulties, especially de novo postoperative UR. Other authors have reported rates of UR of 7�C21% and clotrelated UR of 0�C5% after HoLEP. In the present study, these rates were 11.0% and 7.1%, respectively.