Therefore, in this study, we decided to Tulathromycin B verify the effects of perioperative antioxidative responses of platelets on post-transplant renal allograft function in humans. In our study, we observed several significant differences in platelet antioxidative activity, which varied according to the patient��s post-transplant outcome. Namely, we noticed that both platelet pathways of hydrogen peroxide neutralization seem to be less efficient in patients with post-transplant graft reactivation problems than in EGF patients. Lower activity of catalase and GST was observed in the SGF/DGF groups; these groups also showed significant lowering of G6P action during the reperfusion period. Several recent papers have demonstrated that excessive free radical generation promotes vasoconstriction and plays a key role in the development of renal injury that may be prevented by overexpression of catalase, which modulates the intrarenal GSK 650394 reninangiotensin system. Our study indirectly supports these observations, as catalase activity was strongly associated with posttransplant kidney allograft function and perioperative activity of catalase was significantly lower in patients having problems with postoperative allograft reactivation. Platelet antioxidative enzymes were negatively associated with perioperative systemic isoprostane levels; concentrations of these enzymes significantly increased during the reperfusion time in all groups and were higher in DGF individuals in the fifth minute of reperfusion. Thus, our experiments highlight that during renal transplantation, intensified oxidative stress always occurs; however, this stress is most evidently pronounced in DGF individuals. Moreover, we hypothesize that during the early phase of kidney allograft reperfusion, platelet antioxidants probably exert a protective effect by modulating the perioperative intensity of oxidative stress that accompanies the reperfusion period following renal transplantation. This potential protection offered by platelets seems to be less expressed in SGF/DGF patients and probably clinically translates to poorer post-transplant allograft function. However, the results of our study are based on a relatively small number of patients and may be underpowered. In order to fully verify the proposed hypothesis and derive definite conclusions, further studies are required. These studies could benefit from our analysis, which offers valuable information on the appropriate sample size necessary for reliable verification of the observed phenomena. In addition, other plausible clinical factors that could be responsible for observed tendencies must also be taken into account, including organ quality, which may increase the risk of poorer post-transplant allograft function. The results of our study support previous observations that clinical parameters such as duration of cold ischemia time and/or pre-transplant hemodialysis treatment may unfavorably influence early post-transplant allograft function.