The RNA-Seq analysis identified several groups of TFs most relevant for microglia activation. Roles have been established for most of these TFs, in macrophage activation. In addition to nf��b and stat we identified additional TFs, whose roles in microglia activation have not yet been established. We observed the significant up-regulation of stat 1 and stat 3 after 4 h in LPS-stimulated BV-2microglia. Similar observations were also observed in peripheral with LPS, revealing the strong upregulation of stat1/stat3 signaling. The RNA-Seq data also revealed that other L-Arginine hydrochloride members of these transcription factor families, could play significant roles in microglia development and activation. However unaffected by LPS, suggesting the highly selective induction of TFs through LPS in BV-2 microglial cells. Nevertheless further studies are warranted to assess earlier timepoint transcription PF-573228 factors profiling as well as how these TFs participate in innate immunity in microglial cells. Furthermore, we confirmed the expression of key inflammation and immunity-related genes as well as cytokines/chemokines in the supernatants were significantly induced in LPS treated primary microglial cells. To further delineate conserved transcription factor-binding motifs, we performed TF motif analysis on LPS-stimulated genes in BV-2 microglial cells. The core promoters of co-expressed genes can be evaluated for over represented cis-regulatory elements after partitioning into suitable modules. Among the two ranges available in Ps can that are closest to this region of interest, the range was selected for the analyses. The promoters of differentially expressed genes revealed the enrichment of DNA sequences not only for NF-��B transcription factors but also forirf1, stat1, stat3 and specificity protein1. These analyses converged the first insights into 5 TF binding motif that can be involved in regulating subset specific genes in BV-2microglial cells. To further elucidate the functional categories for LPS-stimulated inflammatory genes in BV-2 microglial cells, we performed the first functional analysis of the transcripts, isoforms and TSSs.