This method is based on biometric evaluations with fixed doses of the compound to be tested. Accordingly, the starting dose level should be that which is most likely to produce mortality in some of the dosed animals. Therefore, for the present study the starting dose was decided to be 2000 mg/kg body weight. Based on the results, downstream methodology of OECD was followed. The oral acute toxic class method was developed as an alternative to replace the oral LD50 test. It is a stepwise procedure that does not intend to allow calculation of an exact LD50 for a substance, but does allow determination of defined concentration ranges where lethality may be expected. By utilising this approach, sufficient information is obtained on the acute toxicity of the test substance to enable its classification simultaneously reducing the number of animals used for testing. Based on the results of toxicity experiments using two doses i.e 300 and 2000 mg/kg bw, the guidelines allowed extrapolation of data and the LD50 cut-off value could be determined. The LD50 cut-off value of SCD-1 was established to be 2000 mg/kg bw. It was, concluded that SCD-1 for treating Nuciferin aspergillosis in animals was quite safe. The compound was classified into category IV of GHS, this system of classification was developed to increase the consistency among experimental setups in different nations. Seidle et al emphasised that in acute toxicity studies, the classification and labelling of substances is most important. SCD-1 demonstrated protection against infection by A. fumigatus in an intranasal murine model of aspergillosis. The administration of A. fumigatus through intranasal route has been reported to mimic the natural sinopulmonary route of infection in humans. The animals were observed up to 14 days. To prevent unnecessary discomfort, all the animals were euthanized by the 14th day, as the weight of infected-untreated animals Hypaconitine decreased to approximately 20% of the initial body weight. It was observed that mice without any antifungal treatment showed 22.3% survival whereas the animals receiving 200 mg/kg bw of SCD-1, orally, showed a survival rate of 77.8%.