Recover a fast restart of translation is facilitated by increasing a local concentration of translation initiation and elongation components in SGs, where only the key regulator, eIF2a factor, is missing and recruited after a stress Folinic acid calcium salt pentahydrate relief only. There is a supporting evidence from mammalian cells where a phospho-variant of the eIF2a factor subunit was found to be recruited to disassembling SGs and considered as important for SGs disassembly. On the contrary, we found that the eIF2a factor accumulation on SGs does not depend on the phosphorylation status of this factor. It implies that the eIF2a factor is recruited to dissolving SGs also in its unphosphorylated state, thus translation competent. Taken together, it reinforces the hypothesis that SGs serve as sites where translation is effectively initiated at the time of a stress relief. We showed here that a portion of the key translation initiation factor eIF2a is recruited to dissolving SGs, but some of the Sui2-GFP foci did not co-localize with SGs markers in these cells. We suggest that these particular Sui2-GFP foci may represent the eIF2B bodies. In accordance with our assumption that translation is restored on dissolving SGs, the eIF2B bodies should also be formed under recovery from the stress. The eIF2B bodies most probably serve as sites, where guanine nucleotide exchange of the eIF2a factor takes place and the eIF2a-GDP form is converted to the translation competent eIF2a-GTP form. The eIF2B bodies would then help to LOUREIRIN-B regenerate efficiently the translation competent form of the eIF2a factor as suggested previously. The eIF2a-GTP form would then be recruited to sites on dissolving SGs. Altogether, our data support the current view that the composition of stress granules depends on the type and the intensity of the applied stress. We confirmed that formation of yeast heat shock-induced SGs is not dependent on the translation initiation arrest caused by phosphorylation of eIF2a and we propose that translation machinery in heat shocked-cells seems to be primarily modulated at the level of translation elongation since also some translation elongation and termination factors accumulate within SGs. Our data further indicate that SGs reflect the sites where translation initiates after a stress relief. We also show that RNP accumulations formed upon heat shock at 42uC and containing translation elongation and termination factors may develop into genuine SGs upon robust heat shock at 46uC. Although we confirmed that all these accumulations depend on mRNA released from translation, links between heat-induced repression of translation and SGs assembly still remain to be elucidated. The lung originates from the primitive foregut early in the development of land dwelling organisms, and through a complex interplay of signaling molecules the future airway epithelium and surrounding mesenchyme develop into the highly structured arbor-like bronchial-vascular tree. Normal development in mammals occurs in a relative hypoxic environment, which is beneficial for lung organogenesis. Cellular responses to different levels of oxygen are important for development and homeostasis, and the most important oxygen-sensing mechanism to protect cells from oxygen toxicity is the transcriptional response mediated by Hypoxia Inducible Factors, which are also expressed in the lungs.