These genes were divided into different classes as presented in Figures 7, S16, S17, and S18. The major classes of transcription factors genes which showed differential expression in our study were Myb, NAC, basic helix-loop-helix, WRKY, and zinc finger transcription factors. Transcription factors of these types been reported to affect cell wall integrity in other plant species. MYB domain containing transcription factors are CGK 733 involved in secondary cell wall biosynthesis, pollen wall composition, mucilage deposition, extrusion and lignin deposition in Arabidopsis. The MYB46 and MYB83 transcription factors are thought to NSI-189 regulate secondary wall biosynthesis in Arabidopsis. A basic helix-loop-helix transcription factor had been reported to play an important role in tapetal cell development. The role of a MADS-box transcription factor as RIPENING INHIBITOR is in cell wall metabolism and carotenoid biosynthesis and a basic leucine zipper domain containing transcription factor had been reported to affect pollen coat patterning. As demonstrated by Wang et al., WRKY transcription factors are involved in regulation of downstream genes encoding the NAM, ATAF1/2, and CUC2 and CCCH type zinc finger TFs that activate secondary wall synthesis. NAC domain containing transcription factors were reported to be involved in secondary cell wall biosynthesis and in the regulation of cellulose and hemicellulose biosynthetic genes in addition to those involved in lignin polymerization and signaling in Arabidopsis and Eucalyptus. Rice and maize SWNs and MYB46 had been reported as master transcriptional activators of the secondary wall biosynthetic program. The AP2 domain containing family is an ethylene responsive group of transcription factors, and in Arabidopsis thaliana, these were expressed in specific cell types of roots, stems and seeds that undergo suberization. The TT1 genes were another class of the significantly differentially expressed genes in this study.These transcription factor genes had been reported to be involved in seed coat pigmentation and integrity in Arabidopsis. It is likely that the defective seed coat mutation in soybean sets in motion changes in many pathways related to the cell wall.