If the nutritional consequences of eating a food are not apparent until after a meal is processed and absorbed. The animal has to learn from the experience of having eaten a food by associating post-ingestive nutritional consequences with properties of the food, ABT-263 923564-51-6 however this take some times as biotin deficiency is not instantaneous but is steadily rising from day to day. In our study, ants confined to an egg white diet, might increase their intake from day to day to also provide limiting biotin, constraining them as a result to ingest more avidin and enter a deadly loop. Here, we have shown that in ants, the effects of biotin deficiency are not limited to health and performance, but extend to behavior modification and by extension to social organization. Knowing that egg white protein is a common component of ant diets in many laboratories, it might be important to add biotin to synthetic diets to improve ant husbandry. Biotin is a coenzyme required for all forms of life, feeding avidin or streptavidin causes a biotin deficiency that leads to stunted growth and mortality in numerous insect to which we can now add ants. Proteins that bind to vitamins, such as biotin, had been shown to represent potential pest-resistance transgene products. The avidin gene has recently been incorporated into genetically modified crop plants, which are then insecticidal to a variety of insects and aphids. However, many aphid pests of major plant crops are attended or attacked by ants. In light of our results, it might be important to look at the ecological significance of aphids as carriers of avidin from transgenic plants to ant colonies. Traditional Chinese medicine employs compounds called Chinese herbal formulas. In the past three decades, these compounds have been attracting increasing attention for their complementary therapeutic effects to western medicines. CHFs are comprised of multiple components and affect numerous targets, yet the bioactive components of most CHFs have not been elucidated. In the Pharmacopoeia of the People’s Republic of China, only a few components, or even a single component, based on their content in herbs instead of their clinical activities, were controlled in current CHF quality standards. In order to better guarantee the clinical safety and effectiveness of CHF, the bioactive components evidenced by suitable methods should be controlled. Bioactivity control, which reflects information directly related to safety and effectiveness, is indispensable in CHF quality control. Therefore, a change from traditional evaluation methods that emphasize chemical characteristics to a method that quantifies biological effects is urgently required. According to the theory of modern chemical biology, drug effects are achieved by the regulation of biomacromolecules by small molecules in it. Hence, there must be a group of bioactive components responsible for a drug’s clinical effects. The concept of relevance between drug components and effects was first introduced in 2002, and the earliest methodology to match drug effects with prominent peaks in high-performance liquid chromatography fingerprints was reported in 2000. Kong et al. studied the relationship between the HPLC fingerprint and the antibacterial effects of artificial Calculus bovis with the help of chemometric methods.